What is the lowest reasonable melting temperature for PCR primers? - primer annealing calculator
... Taq polymerase used. Temp default extension of 72 degrees. I had a brain fart and ordered primers with a melting point of 40 degrees (if you're by the rule of 2 / 4) and I'm not sure if this is the annealing 30something and see what happens, or new primers, which longer have to.
Tuesday, December 8, 2009
Primer Annealing Calculator What Is The Lowest Reasonable Melting Temperature For PCR Primers?
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